AccQ•Tag Ultra, AccQ•Tag, and Pico•Tag Sample Preparation for Accurate Amino Acid Composition Analysis
AccQ•Tag Ultra, AccQ•Tag and Pico•Tag chemistries are the first step in enabling your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and many other sample matrices. Combined with traditional HPLC, UHPLC, or the Waters ACQUITY UPLC, you can now have a system that is customized to separate amino acids from protein hydrolysates, cell culture media, and food/feed samples to get enhanced resolution. The end result is reliability with precise qualitative and quantitative results.
AccQ•Tag Ultra, AccQ•Tag and Pico•Tag chemistry kits include: ◾Pre-column derivatization reagents ◾Dedicated reversed-phase columns QC tested for amino acid analysis ◾Eluents and standards ◾Documentation on methods for the analysis of these important biocompounds
AccQ•Tag Ultra and AccQ•Tag Derivitization Chemistry
This line of products is a comprehensive, fully tested set of reagents, columns, eluents and methods.
AccQ•Tag is optimized for use with HPLC/UHPLC
AccQ•Tag Ultra is optimized for use with the UPLC Amino Acid Analysis Application Solution ◾Both methods are based on the AccQ•Tag pre-column derivatization method ◾Primary and secondary amino acids are reacted with a single reagent (AccQ•Fluor) in a high-throughput batch process resulting in exceptionally stable derivatives ◾AccQ•Tag Ultra provides high-resolution separations achieved using the AccQ•Tag Ultra UPLC column (1.7 µm particles) and mobile phases ◾Derivatized amino acids resolved and quantified to sub-pmol levels with single wavelength UV detection
Pico•Tag Derivitization Chemistry for HPLC
The Pico•Tag method is applicable to many samples including protein hydrolysates, physiologic fluids, feeds, foods, and pharmaceutical preparations. Pre-column derivatization relies on the coupling reaction of the well-known Edman Degradation, the reaction of phenylisothiocyanate (PITC) with both primary and secondary amino acids to form phenylthiocarbamyl (PTC) derivatives The PTC-amino acid adducts are stable and easily separated by reversed-phase HPLC. A single product is formed for each amino acid. ◾The Pico•Tag Amino Acid Analysis Column is a high-efficiency column specifically certified for use with the Pico•Tag method. This HPLC column separates the amino acid derivatives produced by phenylisothiocyanate (PITC) derivitization. ◾The Pico•Tag workstation is required to perform the Pico•Tag methodology. The workstation and parts can be purchased through Eldex by clicking the following link: ◾Pico•Tag workstation